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小居的分子醫學科學與棒球生活筆記

Molecular cloning

DNA preparation

Polymerase chain reaction (PCR) reaction

  • Polymerase Enzyme types

  • 1. Taq polymerase

  • 2. Pfu II ultra

  • 3. KOD plus

  • 4. Phusion

DNA blunting methods

DNA electrophoresis

  • TAE buffer

  • Agarose powder

  • Agarose Gel stained dye

  • DNA ladder (Marker)

  • DNA loading dye


Restriction enzyme method

Gibson assembly

primer design